Journal
JOURNAL OF NEUROTRAUMA
Volume 38, Issue 12, Pages 1620-1631Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/neu.2020.7373
Keywords
contusion; MRI; neuropathology; tractography; traumatic axonal injury; traumatic brain injury
Funding
- NIH National Institute of Neurological Disorders and Stroke [U01NS086090, K08NS114170, R21NS109627, RF1NS115268, K23NS094538, R01NS0525851, R21NS072652, R01NS070963, R01NS083534, U01NS086625, U24NS10059103, R01NS105820]
- NIH Director's Office [DP2HD101400]
- NIH National Institute for Biomedical Imaging and Bioengineering [P41EB015896, 1R01EB023281, R01EB006758, R21EB018907, R01EB019956]
- NIH National Institute on Aging [R56AG064027, R01AG064027, R01AG008122, R01AG016495]
- NIH National Institute of Diabetes and Digestive and Kidney Diseases [R21DK108277]
- United States Department of Defense [W81XWH-14-2-0176, W81XWH-18-2-0042, W81XWH-15-9-0001]
- James S. McDonnell Foundation
- Abbott Point of Care Inc [A133407]
- National Football League Scientific Advisory Board [131769A]
- Tiny Blue Dot Foundation
- Nancy and Buster Alvord endowment
- NIH [S10RR023401, S10RR019307, S10RR023043]
- BRAIN Initiative Cell Census Network [U01MH117023]
- NIH Blueprint for Neuroscience Research [5U01-MH093765]
- One Mind
Ask authors/readers for more resources
Utilizing ex vivo diffusion MRI technology and histopathological validation, this study found varying degrees of tract disruptions in the cerebral white matter regions of patients with TBI, with pathological features including white matter injury and a strong correlation between tract disruption and density of APP-positive axonal swellings and neurofilament loss.
Diffusion tractography magnetic resonance imaging (MRI) can infer changes in network connectivity in patients with traumatic brain injury (TBI), but the pathological substrates of disconnected tracts have not been well defined because of a lack of high-resolution imaging with histopathological validation. We developed an ex vivo MRI protocol to analyze tract terminations at 750-mu m isotropic resolution, followed by histopathological evaluation of white matter pathology, and applied these methods to a 60-year-old man who died 26 days after TBI. Analysis of 74 cerebral hemispheric white matter regions revealed a heterogeneous distribution of tract disruptions. Associated histopathology identified variable white matter injury with patchy deposition of amyloid precursor protein (APP), loss of neurofilament-positive axonal processes, myelin dissolution, astrogliosis, microgliosis, and perivascular hemosiderin-laden macrophages. Multiple linear regression revealed that tract disruption strongly correlated with the density of APP-positive axonal swellings and neurofilament loss. Ex vivo diffusion MRI can detect tract disruptions in the human brain that reflect axonal injury.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available