Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 400, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2020.123165
Keywords
Iris lactea var. chinensis; Cd tolerance and accumulation; Proteomics; Lignin biosynthesis; Mannose-specific lectin
Categories
Funding
- National Natural Science Foundation of China [41907135]
- Natural Science Foundation of Jiangsu Province [BK20180317, BK20180314]
- Open Fund of Jiangsu Key Laboratory for Research and Utilization of Plant Resources [JSPKLB201843]
- Social Developmental Project of Jiangsu Province [BE2018715]
- Jiangsu Provincial Marine Science and Technology Innovation Special Project [HY2018-5]
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Cadmium pollution has become a serious environmental problem. Iris lactea var. chinensis showed strong Cd tolerance and accumulation ability, which has significant potential to be applied for the phytoremediation of Cd-contaminated soil. However, the lack of molecular information on the mechanism of I. lactea response to Cd limited the improvement of phytoremediation efficiency. In this study, label-free proteomics analysis of Cd response in I. lactea showed that there were 163 and 196 differentially expressed proteins (DEPs) in the shoots and roots, respectively. Bioinformatics analysis indicated the DEPs responding to Cd stress mainly involved in signal transduction, ion transport, redox etc., and participate in the pathway of amino acid biosynthesis, lignin biosynthesis, glycerolipid metabolism and glutathione metabolism. Besides, differential expression of seven DEPs was validated via gene expression analysis. Finally, we found that a Cd-induced mannose-specific lectin (IlMSL) from I. lactea enhanced the Cd sensitivity and increased Cd accumulation in yeast. The results of this study will enhance our understanding of the molecular mechanism of Cd tolerance and accumulation in I. lactea and ultimately provide valuable resources for using Cd tolerant genes for developing efficient strategies for phytoremediation of Cd-contaminated soils or limiting Cd accumulation in food crops.
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