A novel approach for treating acid mine drainage through forming schwertmannite driven by a mixed culture of Acidiphilium multivorum and Acidithiobacillus ferrooxidans prior to lime neutralization

As the predominant treatment approach of acid mine drainage (AMD), lime neutralization often exhibits in-efficiencies since the abundance of iron and sulfate in AMD usually form iron hydroxide and gypsum precipitate coatings on the surface of lime. In this study, a novel approach of biomineralization prior to lime neutralization for treating AMD was proposed, in which iron and sulfate were biologically precipitated as schwertmannite through iron biological reduction-oxidation driven by a culture mixed with Acidiphilium multivorum JZ-6 and Acidithiobacillus ferrooxidans LX5. It was found that only five cycles of iron reduction by A. multivorum JZ-6 followed by iron oxidation by A. ferrooxidans LX5 could remove completely iron and nearly 40% of sulfate in AMD, while non-ferrous metals (Al, Mn, Cu, Ni, and Zn) were hardly removed. Consequently, the amounts of lime required and sludge generated in the subsequent lime neutralization process were reduced by 56% and 68%, respectively. As a result, the content of non-ferrous metals in the sludge was increased by 3.2 folds. The level of Al was increased surprisingly to 19% (wt/wt), a level similar to the commercially valuable bauxite. The novel process of biomineralization prior to lime neutralization provides a sustainable way for AMD treatment.