Journal
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
Volume 58, Issue 11, Pages 4205-4216Publisher
SPRINGER INDIA
DOI: 10.1007/s13197-020-04894-5
Keywords
Pharmabiotics; Lactobacillus acidophilus LA-5; Food-grade medium; Response surface methodology
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This study aimed to optimize the co-production of conjugated linoleic acid (CLA), exopolysaccharides (EPSs) and bacteriocins (BACs) by Lactobacillus acidophilus LA-5, achieving high viable cell number, CLA, EPSs, and inhibition activity at optimal conditions. The analysis revealed the functional groups of pharmabiotics and showed the presence of CLA isomers and the composition of EPSs. SDS page analysis reported BACs molecular weight fractions, demonstrating the potential for simultaneous production of bioactive metabolites by probiotics in supplemented cheese whey.
This study aimed to optimize the co-production of conjugated linoleic acid (CLA), exopolysaccharides (EPSs) and bacteriocins (BACs) by Lactobacillus acidophilus LA-5 in dairy food-grade by-product. The factorial design revealed that the significant factors were temperature, time, and yeast extract. Then the response surface methodology was used for optimization. At the optimal conditions the viable cell number, CLA, EPSs, and inhibition activity were 2.62 +/- 0.49 9 x 10(8) CFU/mL, 51.46 +/- 1.50 mu g/mL, 348.24 +/- 5.61 mg/mL and 12.46 +/- 0.80 mm, respectively. FTIR, GC, TLC, and SDS page analysis revealed the functional groups of pharmabiotics. The FTIR, GC, TLC, and SDS page analysis showed that both CLA isomers (c-9, t-11, and t-10, c-12) produced. The FTIR, GC, TLC, and SDS page analysis indicated that produced EPSs were composed of glucose, mannose, galactose, xylose, and fructose. FTIR, GC, TLC, and SDS page used to report BACs molecular weight, which showed two fractions by molecular mass 35 and 63 kDa. Previously the ability of different probiotic bacteria investigated and optimized the production of CLA, EPSs, and BACs, but, there was no report on the co-producing capacity of these bioactive metabolites by probiotics. The present work was investigated to optimize the co-production of pharmabiotic metabolites by L. acidophilus LA-5, in supplemented cheese whey as a cultivation medium.
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