Journal
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
Volume 58, Issue 12, Pages 4514-4523Publisher
SPRINGER INDIA
DOI: 10.1007/s13197-020-04933-1
Keywords
Extraction yield; Total phenolic compound content; Fatty acids; Caffeine; 5-Caffeoylquinic acid
Categories
Funding
- CONACYT [CB-2015-01-258118, FORDECYT 292474, 413405]
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In this study, a supercritical CO2-ethanol extraction method was optimized to obtain green coffee oil rich in bioactive compounds. The extraction yield and total phenolic compound content of green coffee supercritical extract (GCSE) were evaluated using a central composite design, showing that pressure and cosolvent content significantly affected yield, while temperature and cosolvent content impacted total phenolic compound content. The optimal conditions predicted an extraction yield of 7.7% with a total phenol content of 5.4 mg gallic acid equivalent g GCSE(-1), indicating the high chemical quality and antioxidant potential of GCSE for functional ingredient use.
In this research, a supercritical CO2-ethanol extraction was optimized to obtain a green coffee oil rich in bioactive compounds. A face-centered central composite design was used to evaluate the effect of temperature (50-70 degrees C), extraction pressure (15.0-30.0 MPa), and cosolvent content (5-20%) on the extraction yield and total phenolic compound content of green coffee supercritical extract (GCSE). The experimental data were fitted to a second-order polynomial model. According to the statistical analyses, the lack of fit was not significant for either mathematical model. From the response surface plots, the extraction pressure and cosolvent content significantly impacted the extraction yield, while the total phenolic compound content was impacted by temperature and cosolvent content. The optimal conditions were a 20% cosolvent content, a pressure of 30 MPa, and a temperature of 62 degrees C, which predicted an extraction yield of 7.7% with a total phenol content of 5.4 mg gallic acid equivalent g GCSE(-1). The bioactive compounds included 5-caffeoylquinic acid (11.53-17.91 mg g GCSE(-1)), caffeine (44.76-79.51 mg g GCSE(-1)), linoleic acid (41.47-41.58%), and palmitic acid (36.07-36.18%). Our results showed that GCSE has the outstanding chemical quality and antioxidant potential, suggesting that GCSE can be used as a functional ingredient.
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