Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 84, Issue 3, Pages 207-211Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2015.10.017
Keywords
Tuberculosis; Pyrazinamide; Resistance; pncA; rpsA
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Funding
- Infectious Diseases Special Project, Minister of Health of China [2012ZX10003002]
- Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [ZYLX201304]
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Our study was aimed to identify the phenotypic and genotypic pyrazinamide (PZA) resistance features among multidrug-resistant (MDR) isolates in a national tuberculosis (TB) referral center of China. PZA susceptibility test was performed for a total of 142 MDR-TB clinical isolates using the MGIT 960 PZA kits, and the pncA, rpsA, and panD genes were sequenced. Extensively drug-resistant (XDR) and pre-XDR strains had higher PZA resistance rate than that of MDR strains which were still sensitive to fluoroquinolone and aminoglycoside (42.9%, 24/56) (chi(2) = 8.922, P = 0.012). No panD mutation was detected among the PZA resistant strains with wild type pncA and rpsA genes. Our study indicates that PZA-resistant frequency increases with TB drug resistance level; pncA, rpsA, and panD mutations had strong, low, and no correlation with PZA resistance, and rapid molecular assay will facilitate the timely identification of the PZA-sensitive MDR-TB. (C) 2016 Elsevier Inc. All rights reserved.
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