Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 84, Issue 4, Pages 318-321Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2015.12.009
Keywords
Mycobacterium kansasii; tuf; Subtyping; PCR restriction enzyme analysis
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Funding
- National Centre for Research and Development: LIDER Programme [LIDER/044/457/L-4/12/NCBR/2013]
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Within this study, a new, rapid method for subtyping of Mycobacterium kansasii was developed based on the sequence analysis of the tuf gene coding for the Tu (thermo-unstable) elongation factor (EF-Tu). The method involves PCR amplification of ca. 740-bp tuf gene fragment, followed by digestion with the Mval restriction endonuclease. (C) 2016 Elsevier Inc. All rights reserved.
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