Enhanced Antibacterial Potential of Naringin Loaded β Cyclodextrin Nanoparticles

beta-cyclodextrin (beta-CD) is biocompatible and biodegradable polymer having inherent hydrophobic cavities, thus is widely used as a drug nanocarrier for less water soluble drugs. Naringin is a pharmacologically active important compound however, its therapeutic efficacy is limited due to its unique physicochemical properties including lower aqueous solubility. This study reports improvement in bactericidal potentials of naringin through construction of its nano-formulation using biocompatible beta-CD. Naringin loaded beta-CD NPs were prepared via solvent evaporation method and characterized using various techniques such as FTIR, UV-visible spectroscopy, AFM, DSC and TGA analysis. Tetrazolium Microplate and AFM assays were used for investigating the bactericidal potential of naringin loaded beta-CD NPs. Monodispersed NPs with spherical morphology of 70.00 +/- 15.06 nm mean diameter were obtained that possessed net negative surface charge of - 18.20 +/- 3.24 mV and were highly stable and did not change the chemical nature of the drug in various media. Antibacterial activity of naringin in beta-CD NPs significantly enhanced that was authenticated by AFM, showing prominent destructive changes in the bacterial cell's morphology through destabilization of outer membrane of bacterial cells by drug loaded NPs. Results of this study suggest that the beta-CD NPs of naringin could be used as a successful formulation for developing more efficacious form of the drug against various bacterial infections.

Automated summary

The study improved the bactericidal potentials of naringin by constructing its nano-formulation using biocompatible beta-CD, resulting in enhanced antibacterial activity and potential for developing more efficacious drugs against bacterial infections.