Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 59, Issue 3, Pages -Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02078-20
Keywords
Blastomyces; ecology; epidemiology; molecular assay; validation
Categories
Funding
- WC, New York State Department of Health (NYSDOH)
- Centers for the Disease Control and Prevention (CDC) [NU50CK000516]
- National Science Foundation [DBI1757170]
Ask authors/readers for more resources
A highly sensitive, specific, and reproducible TaqMan duplex real-time PCR assay was developed to differentiate Blastomyces dermatitidis and Blastomyces gilchristii, revealing the distribution of these pathogens in blastomycosis cases in New York.
Blastomycosis due to Blastomyces dermatitidis and Blastomyces gilchristii is a significant cause of respiratory mycoses in North America with occasional reported outbreaks. We developed a highly sensitive, specific, and reproducible TaqMan duplex real-time PCR assay for the differentiation of B. dermatitidis and B. gilchristii. The new assay permitted retrospective analysis of Blastomyces cultures (2005 to 2019) and primary clinical specimens from blastomycosis cases (2013 to 2019) from New York patients. We identified B. dermatitidis as the predominant pathogen in 38 cases of blastomycosis, while B. gilchristii was a minor pathogen involved in five cases; these findings expand understanding of blastomycosis in New York. The duplex real-time PCR assay could be implemented in reference and public health laboratories to further understand the ecology and epidemiology of blastomycosis due to B. dermatitidis and B. gilchristii.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available