Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1634, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2020.461674
Keywords
High performance liquid chromatography; Mixed-mode stationary phase; Ionic liquids; Chiral separation
Funding
- National Natural Science Foundation of China [21804112]
- talent introduction program of Southwest Medical University [090300040040]
- project of Department of Education of Sichuan Province [18ZA0527]
- university level fund of Southwest medical university [2017-ZRQN-0118]
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A novel reversed-phase/hydrophilic interaction/ion-exchange (RPLC/HILIC/IEC) and chiral recognition mixed-mode stationary phase material was synthesized using 3-n-octadecyl-1-vinylimidazolium bromide and 6-(1-allylimidazolium)-cyclodextrin tosylate as functional monomers. After identifications of inter mediates and stationary phase materials using nuclear magnetic resonance analysis, Fourier transform infrared spectrometer, element analysis and thermogravimetric analysis, the synthesized Sil-VMBD material was packed into an empty column under high pressure. The mixed-mode retention performance was researched by hydrophobic, hydrophilic and ionic compounds. And the retention mechanism of the multi-mode stationary phase was studied via changing the mobile phase composition and pH value. Moreover, the enantiomers of 1-phenyl-1-propanol, warfarin and styrene oxide were separated rapidly under reverse-phase mode. Fast enantioseparation of ibuprofen and ketoprofen were obtained within 4 min under polar organic mode. The experimental results show that the as-prepared mixed-mode column possesses reversed-phase, hydrophilic and ion-exchange interactions with various analytes, and these interactions also enable the stationary phase with multi-mode and chiral separation abilities. (C) 2020 Elsevier B.V. All rights reserved.
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