Bioactive clerodane diterpenes of giant goldenrod (Solidago gigantea Ait.) root extract

Giant goldenrod (Solidago gigantea Ait.) root extract was screened for bioactive compounds by high-performance thin-layer chromatography (HPTLC), coupled with effect-directed analysis including antibacterial (Bacillus subtilis F1276, B. subtilis subsp. spizizenii, Aliivibrio fischeri and Xanthomonas euvesicatoria), antifungal (Fusarium avenaceum) and enzyme inhibition (acetyland butyrylcholinesterases, alpha- and beta-glucosidases and alpha-amylase) assays. Compounds of six multipotent zones (Sg1-Sg6) were characterized by HPTLC-heated electrospray ionization-high-resolution mass spectrometry (HRMS) and HPTLC-Direct Analysis in Real Time-HRMS. Apart from zone Sg3, containing three compounds, a single characteristic compound was detectable in each bioactive zone. The bioassay-guided isolation using preparative-scale flash chromatography and high-performance liquid chromatography provided eight compounds that were identified by NMR spectroscopy as clerodane diterpenes. All isolates possessed inhibiting activity against at least one of the tested microorganisms. (C) 2020 The Author(s). Published by Elsevier B.V.

Automated summary

The study screened bioactive compounds in Giant goldenrod root extract using HPTLC and identified clerodane diterpenes as the active compounds through NMR spectroscopy. All isolates demonstrated inhibitory activity against at least one of the tested microorganisms.