Short communication Selective Enrichment of Low-abundance Compounds in a Mixture by Capillary Electrophoresis

Recently, we developed a new approach for the selective enrichment of low-abundance compounds in biological samples by capillary electrophoresis. As a model test, the low-abundance compound lysozyme was successfully fractionated from a mixture containing high-abundance compound BSA (1:4500) using a custom-made apparatus. The feasibility of this approach for real complex biological samples was verified by rat serum, wherein three low-abundance proteins with high charge/mass ratios were detected. (C) 2020 Elsevier B.V. All rights reserved.

Automated summary

A new approach for selective enrichment of low-abundance compounds in biological samples by capillary electrophoresis was developed. The method successfully separated low-abundance lysozyme from a mixture containing high-abundance BSA, and was verified in rat serum for detection of low-abundance proteins with high charge/mass ratios.