Journal
JOURNAL OF CHEMICAL PHYSICS
Volume 153, Issue 24, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/5.0033107
Keywords
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Funding
- Swiss National Science Foundation (SNF) through the NCCR MUST [200020B_188694/1]
- Swiss National Science Foundation (SNF) [200020B_188694] Funding Source: Swiss National Science Foundation (SNF)
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Over the last decades, photoreceptive proteins were extensively studied with biophysical methods to gain a fundamental understanding of their working mechanisms and further guide the development of optogenetic tools. Time-resolved infrared (IR) spectroscopy is one of the key methods to access their functional non-equilibrium processes with high temporal resolution but has the major drawback that experimental data are usually highly complex. Linking the spectral response to specific molecular events is a major obstacle. Here, we investigate a cyanobacteriochrome photoreceptor with a combined approach of transient absorption spectroscopy in the visible and IR spectral regions. We obtain kinetic information in both spectral regions by analysis with two different fitting methods: global multiexponential fitting and lifetime analysis. We investigate the ground state dynamics that follow photoexcitation in both directions of the bi-stable photocycle (Pr-* and Pg(*)) in the nanosecond and microsecond time regimes. We find two ground state intermediates associated with the decay of Pr-* and four with Pg(*) and report the macroscopic time constants of their interconversions. One of these processes is assigned to a structural change in the protein backbone.
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