Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
Volume 109, Issue 7, Pages 1017-1028Publisher
WILEY
DOI: 10.1002/jbm.b.34766
Keywords
dental implants; nano-LC-MS/MS; roughness; salivary pellicle; titanium; wettability
Funding
- UNAM-PAPIIT [IA206418, IA208220, IT200319]
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This study revealed differences in protein composition of salivary pellicles formed on Titanium surfaces with different roughness and wettability, indicating that the physicochemical characteristics of the implant surfaces influence protein adsorption and composition.
This study reports the differences in the protein composition of salivary pellicles formed under in situ conditions on two Titanium (Ti) surfaces, with different roughness and wettability. Smooth pretreatment Ti surfaces (Ti-PT) with an average roughness (Ra) of 0.45 mu m and a water contact angle (WCA) of 92.4 degrees, as well as a more rough sandblasted, large grit, acid-etched treatment Ti surfaces (Ti-SLA) with a Ra of 3.3 mu m and WCA of 131.8 degrees, were tested. The salivary pellicles were quantitatively analyzed by bicinchoninic acid assays, and the protein identification was performed by Nano-LC-MS/MS (nano mass spectrometry). Protein levels of 2.5, and 9.1 mu g/ml were quantified from the detached salivary pellicle formed on the Ti-PT and Ti-SLA surfaces, respectively. Using Nano-LC-MS/MS, a total of 597 proteins were identified on all the substrates tested; 43 proteins were identified only on the Ti-PT, and 226 proteins were adsorbed solely on the Ti-SLA substrates. The physicochemical characteristics of the Ti implant surfaces modified the amount and the identity of the salivary proteome of the pellicles formed, confirming the high selectivity of the protein pellicle formed on a surface once is exposed in the oral cavity.
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