Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 69, Issue 2, Pages 698-703Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c07020
Keywords
cyanobacteria; biocontainment; farnesene; CO2 conversion; CCM
Funding
- Individual Research Program through the National Research Foundation of Korea - Korean Government (Ministry of Science, ICT) [2018R1D1A1B07049415, 2020R1F1A1048292]
- Ministry of Oceans and Fisheries [213008-05-5-SB910]
- National Research Foundation of Korea [2018R1D1A1B07049415, 2020R1F1A1048292] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
This study developed a biocontainment system using genetically modified cyanobacteria to produce alpha-farnesene, by deleting gene clusters related to the beta-carboxysome and the carbon concentrating mechanism. The production of alpha-farnesene was dependent on CO2 concentration in the photobioreactor, with potential applications in next generation bio-solar cell factories.
Biocontainment systems have been developed to mitigate the concerns regarding biosafety and environmental risk because of the possible escape of genetically modified organisms into the environment following large-scale outdoor cultivation. Here, we present a biocontainment system entailing genetically modified Synechococcus elongatus PCC 7942, also engineered for alpha-farnesene production using a de-evolutionary strategy. In this approach, the gene cluster encoding the beta-carboxysome and the associated carbon concentrating mechanism (CCM) were deleted in the alpha-farnesene-producing cyanobacteria, resulting in no cell growth and no alpha-farnesene production at ambient CO2 concentrations (100% air bubbling). However, cell growth and alpha-farnesene production were detected in the CCM-deficient strains at high CO2 concentrations (5% CO2 [v/v], 10% CO2 [v/v]), albeit at levels lower than those of the parental control. To overcome this limitation, the overexpression of carbonic anhydrase and bicarbonate transporter genes in the CCM-deficient strains restored cell growth and the production level of alpha-farnesene (5.0 +/- 0.6 mg/L) to that of the parental control. The production of alpha-farnesene in the later strains strictly depended on CO2 concentration in the photobioreactor and did not rely on a chemical induction process. Thus, next generation bio-solar cell factories could be promoted with the suggested biocontainment system.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available