Mucilaginibacter mali sp. nov., isolated from rhizosphere soil of apple orchard

A novel Gram-negative bacterium, designated G2-14(T), was isolated from rhizosphere soil sample collected from apple orchard in Chungju-si, Chungcheongbuk-do, Republic of Korea. Strain G2-14(T) was a strictly aerobic, non-spore-forming, non-motile and short-rod-shaped bacterium. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain G2-14(T) was closely related to Mucilaginibacter myungsuensis HMD1056(T) (96.9 %) and Mucilaginibacter boryungensis BDR-9(T) (96.8 %). The major cellular fatty acids (>10%) of strain G2-14(T) were summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c) and iso-C-15:0. The predominant quinone and the major polar lipid were menaquinone-7 and phosphatidylethanolamine, respectively. Strain G2-14(T) produced acetic acid. The DNA G+C content based on whole genome sequences was 46.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain G2-14(T) represents a novel species in the genus Mucilaginibacter, for which the name Mucilaginibacter mali sp. nov. is proposed. The type strain is G2-14(T) (=KCTC 72533(T)=NBRC 114179(T)).

Automated summary

The novel Gram-negative bacterium G2-14(T) isolated from apple orchard rhizosphere soil in Chungju-si, South Korea, is closely related to other strains in the genus Mucilaginibacter. G2-14(T) produces acetic acid and has unique cellular fatty acids.