Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/ijms22010148
Keywords
genetically-encoded fluorescent sensors; FRET sensors; in vivo imaging; CRISPR techniques; high-throughput screening (HTS); anti-cancer drug screening; G Protein-Coupled Receptor (GPCR) modulators; Genetically Encoded Voltage Indicators (GEVI); Genetically Encoded Calcium Indicators (GECI)
Funding
- Russian Science Foundation (RSF) [17-15-01175]
- Ministry of Science and Higher Education of the Russian Federation [075-15-2019-1789]
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Genetically-encoded fluorescent sensors have been actively developed and are beneficial for real-time monitoring and high reproducibility in drug screening. Their advantages extend to whole organism drug screening models and the potential combination with CRISPR techniques for human disease modeling.
Genetically-encoded fluorescent sensors have been actively developed over the last few decades and used in live imaging and drug screening. Real-time monitoring of drug action in a specific cellular compartment, organ, or tissue type; the ability to screen at the single-cell resolution; and the elimination of false-positive results caused by low drug bioavailability that is not detected by in vitro testing methods are a few of the obvious benefits of using genetically-encoded fluorescent sensors in drug screening. In combination with high-throughput screening (HTS), some genetically-encoded fluorescent sensors may provide high reproducibility and robustness to assays. We provide a brief overview of successful, perspective, and hopeful attempts at using genetically encoded fluorescent sensors in HTS of modulators of ion channels, Ca2+ homeostasis, GPCR activity, and for screening cytotoxic, anticancer, and anti-parasitic compounds. We discuss the advantages of sensors in whole organism drug screening models and the perspectives of the combination of human disease modeling by CRISPR techniques with genetically encoded fluorescent sensors for drug screening.
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