4.7 Article

Biochemical Characterization and Function of Eight Microbial Type Terpene Synthases from Lycophyte Selaginella moellendorffii

Journal

Publisher

MDPI
DOI: 10.3390/ijms22020605

Keywords

lycophyte; SmMTPSLs; stress physiology; GC-MS; antibacterial

Funding

  1. National Science Foundation of China [31770333, 31370329, 11631012]
  2. Program for New Century Excellent Talents in University [NCET-12-0896]
  3. Fundamental Research Funds for the Central Universities [GK201403004]

Ask authors/readers for more resources

This study investigated the biochemical functions of SmMTPSLs in Selaginella moellendorffii, identifying their roles in synthesizing monoterpenes and sesquiterpenes, as well as their potential involvement in plant response to stress. Additionally, it demonstrated the antibacterial effects of terpenoids produced by specific SmMTPSLs on Pseudomonas syringae pv. tomato DC3000 and Staphylococcus aureus.
Selaginella moellendorffii is a lycophyte, a member of an ancient vascular plant lineage. Two distinct types of terpene synthase (TPS) genes were identified from this species, including S. moellendorffii TPS genes (SmTPSs) and S. moellendorffii microbial TPS-like genes (SmMTPSLs). The goal of this study was to investigate the biochemical functions of SmMTPSLs. Here, eight full-length SmMTPSL genes (SmMTPSL5, -15, -19, -23, -33, -37, -46, and -47) were functionally characterized from S. moellendorffii. Escherichia coli-expressed recombinant SmMTPSLs were tested for monoterpenes synthase and sesquiterpenes synthase activities. These enzymatic products were typical monoterpenes and sesquiterpenes that have been previous shown to be generated by typical plant TPSs when provided with geranyl diphosphate (GPP) and farnesyl diphosphate (FPP) as the substrates. Meanwhile, SmMTPSL23, -33, and -37 were up-regulated when induced by alamethicin (ALA) and methyl jasmonate (MeJA), suggesting a role for these genes in plants response to abiotic stresses. Furthermore, this study pointed out that the terpenoids products of SmMTPSL23, -33, and -37 have an antibacterial effect on Pseudomonas syringae pv. tomato DC3000 and Staphylococcus aureus. Taken together, these results provide more information about the catalytic and biochemical function of SmMTPSLs in S. moellendorffii plants.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available