Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 21, Issue 22, Pages -Publisher
MDPI
DOI: 10.3390/ijms21228869
Keywords
acyl activating enzyme 3; oxalyl-CoA synthetase; oxalate accumulation; Cd and Al tolerance; wild soybean
Funding
- National Key R&D Program of China [2018YFD0201006]
- China Agricultural Research System [CARS-04-PS09]
- Guangdong Agricultural Research System [2019KJ136]
- Research Project of the State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources [SKLCUSA-b201804]
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Acyl activating enzyme 3 (AAE3) was identified as being involved in the acetylation pathway of oxalate degradation, which regulates the responses to biotic and abiotic stresses in various higher plants. Here, we investigated the role of Glycine soja AAE3 (GsAAE3) in Cadmium (Cd) and Aluminum (Al) tolerances. The recombinant GsAAE3 protein showed high activity toward oxalate, with a K-m of 105.10 +/- 12.30 mu M and V-max of 12.64 +/- 0.34 mu mol min(-1) mg(-1) protein, suggesting that it functions as an oxalyl-CoA synthetase. The expression of a GsAAE3-green fluorescent protein (GFP) fusion protein in tobacco leaves did not reveal a specific subcellular localization pattern of GsAAE3. An analysis of the GsAAE3 expression pattern revealed an increase in GsAAE3 expression in response to Cd and Al stresses, and it is mainly expressed in root tips. Furthermore, oxalate accumulation induced by Cd and Al contributes to the inhibition of root growth in wild soybean. Importantly, GsAAE3 overexpression increases Cd and Al tolerances in A. thaliana and soybean hairy roots, which is associated with a decrease in oxalate accumulation. Taken together, our data provide evidence that the GsAAE3-encoded protein plays an important role in coping with Cd and Al stresses.
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