Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 165, Issue -, Pages 239-248Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.09.187
Keywords
Polysaccharides of Dendrobium devonianum; Nanoparticles; Ovalbumin macrophages; Lymphocytes; Adjuvant activity
Funding
- Natural Science Foundation of China [31602098, 3207190119]
- Scientific research and innovation Team in Henan University of Animal Husbandry and Economy [2018KYTD18]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX20-1494, 2018ZX10711001-003-003]
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Recently, nanoparticles have been widely used in drug and vaccine adjuvant delivery. Dendrobium devonianum Polygonatum(DP), a main biologically active ingredient isolated from Dendrobium devonianum, has been widely used in the clinic as an immunostimulant to stimulate and improve immune responses, contributing to its excellent biological activity. To increase the immune efficacy of DP, macrophage cell membrane-coated drug nanocrystals featuring homologous immune escape, targeting ability and low toxicity are in high demand. In this study, a new drug and vaccine adjuvant delivery system, PEI-MM-PLGA-DP/OVA, was designed and developed. This study aimed to report the macrophage immunomodulatory activity of PEI-modified macrophage cell membrane-coated PLGA nanoparticles encapsulating Dendrobium devonianum polysaccharides. PEI-MM-PLGA-DP/OVA could promote antigen uptake by macrophage and lymphocyte proliferation, increase the expression levels of MHC II, CD80 and CD86, and upregulate the ratio of CD4(+) to CD8(+) T cells in immunizedmice. PEI-MM-PLGA-DP/OVA induced the highest TNF-alpha, IFN-gamma, IL-4, and IL-6 cytokine secretion levels and the levels of OVA-specific antibodies (IgG) compared with the other groups. The above results indicated that PEI-MM-PLGA-DP/OVA had better adjuvant activity than PLGA-DP/OVA and MM-PLGA-DP/OVA. (C) 2020 Published by Elsevier B.V.
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