Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 166, Issue -, Pages 1535-1542Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.11.033
Keywords
IC50; Technical lignin; Radical scavenging; Antibacterial; Antitumoural; Hardwood lignin
Funding
- CNPq - Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (National Counsel of Technological and Scientific Development)
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Kraft lignin can be fractionated into different molecular weights by acid precipitation at different pH values, with lower molecular weight fractions showing better antioxidant and antibacterial activities. Some fractions also exhibit potential cytotoxicity against glioma cells.
Kraft lignin, so far useful for energy generation, has been gathering considerable attention as an alternative material to replace fossil-based resources mainly due to its high phenolic content. However, the wide molecular weight distribution and chemical composition heterogeneity led to the development of fractionation methods. Herein, to narrow such characteristics we used eucalypt kraft lignin fractionated at pH's 9, 7, 5 and 3 by sequential acid precipitation. These lignin fractions were first characterised by simultaneous pyrolysis and trimethylsilylation (SPyT) with N-Methyl-N-(trimethylsilyl) trifluoroacetamide with posterior tests of antioxidant, antibacterial, and antitumour activities. We observed higher ratio of syringyl/guaiacyl groups and increase in antioxidant activity in those fractions with lower molecular weight (precipitated at lower pH's). Fractions precipitated at pH's 9 and 7 have shown an outstanding antibacterial activity against five bacteria. Moreover, fractions 7 and 5 presented at cytotoxicity tests higher ability to inhibit the growth of U87MG and T98G glioma cells, while only a slight inhibition of adult human fibroblasts (non-tumour cells) was detected. (c) 2020 Elsevier B.V. All rights reserved.
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