Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 164, Issue -, Pages 855-862Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.07.011
Keywords
Chitosan; Hydrogel; Thermos-responsive; Resistant bacteria, post-wound infection; Wound dressing
Funding
- Mazandaran University of Medical Sciences [7146]
Ask authors/readers for more resources
In this study, we synthesized thermo-responsive chitosan (TCTS) hydrogels, and loaded with different concentrations of antimicrobial peptide (AMP) (0, 4, 8 and 16 mu g.ml(-1)) to fabricate an antibacterial wound dressing against resistant clinical isolates. Physico-chemical properties, release behavior, cytobiocompatibility and anti-bacterial activity of the AMP-TCTS hydrogels against standard strain and resistant Acinetobacter baumannii were fully determined in vitro. The TCTS-40% beta-glycerolphosphate hydrogels showed a gelation time of 15 min at 37 degrees C. 80% weight loss at day 35 with no changes in pH value was observed. AMP-TCTS hydrogels showed a burst release of AMP (around 40%) at day 1, and a controlled release up to day 7. A dramatic water uptake was observed at first 4 h, and then continued for 10 h in a steady manner. All the AMP-TCTS hydrogels showed excellent cytobiocompatibility for human fibroblasts. The TCTS showed no antibacterial activity against both standard strain and clinical isolates. All the AMP-TCTS hydrogels had strong antibacterial activity against standard strains, but only 16 mu g.ml(-1) showed antibacterial behavior against resistant A. baumannii. Our results strongly suggest the 16 mu g.ml(-1) AMP-TCTS hydrogel as an excellent antibacterial wound dressing against resistant A. baumannii, and now promises to proceed with pre-clinical investigations. (C) 2020 Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available