Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 167, Issue -, Pages 1424-1434Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.11.096
Keywords
Musca domestica; Phormicin; Antimicrobial peptide; Biofilm formation
Funding
- National Natural Science Foundation of China [31760250]
- Guizhou Science and Technology Plan Project (QIAN Science contract) [(2019) 1273]
- Scientific Plan of the Guizhou Provincial Health and Fitness Commission [gzwjkj2015-1-028]
- Gui Yang Bureau of Science & Technology Scientific Project (ZHU science contract) [(20151001) SHE-15]
- Bureau of Science & Technology of Guiyang city [(20161001) 023, (2017)5-26]
- Guizhou Medical University [(20161001) 023, (2017)5-26]
- Doctoral Talents Cultivating Fund of Guizhou Medical University [Academy-PHD-J-2014-018]
- China Scholarship Council [201908520005]
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Housefly-derived Phormicin peptides showed antimicrobial effects against MRSA infections, disrupting biofilm formation and regulating gene networks related to biofilm formation. These findings suggest that Phormicin has potential as a candidate agent for clinical MRSA chemotherapy.
The increasing drug resistance of pathogenic bacteria is a crisis that threatens public health. Antimicrobial peptides (AMPs) have been suggested to be potentially effective alternatives to solve this problem. Here, we tested housefly Phormicin-derived peptides for effects on Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) infections in vitro and in vivo. A decreased bacterial load of MRSA was observed in the mouse scald model after treatment with Phormicin and in the positive control group (vancomycin). A mouse scrape model indicated that Phormicin helps the host fight drug-resistant MRSA infections. The protective effect of Phormicin on MRSA was confirmed in the Hermetia illucens larvae model. Phormicin also disrupted the formation of S. aureus and MRSA biofilms. Furthermore, this effect coincided with the downregulation of biofilm formation-related gene expression (agrC, sigB, RNAIII, altA, rbf, hla, hld, geh and psm?). Notably, virulence genes and several regulatory factors were also altered by Phormicin treatment. Based on these findings, housefly Phormicin helps the host inhibit MRSA infection through effects on biofilm formation and related gene networks. Therefore, housefly Phormicin potential represents a candidate agent for clinical MRSA chemotherapy. (C) 2018 Elsevier B.V. All rights reserved.
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