Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 164, Issue -, Pages 3446-3453Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.08.217
Keywords
Photosynthetic microorganisms; Cyanobacterium; Serine metalloprotease; Thrombosis; Purification
Funding
- Coordenacao e Aperfeicoamento de Pessoal de Nivel Superior Brazil (CAPES) [001]
- Foundation for Science and Technology of the State of Pernambuco (FACEPE) [BFP-0079-5.05/20, APQ-0252-5.07/14]
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Artrhospira (Spirulina) platensis produced fibrinolytic enzyme under mixotrophic conditions using corn steep liquor (CSL). The enzyme was extracted, purified by combination of two chromatographic techniques and biochemically characterized. Maximum fibrinolytic production (268.14 U mg(-1)) was obtained using liquid medium culture composed by 0.2% CLS after 10th day of cultivation. Fibrinolytic activity was higher when extracted by homogenization methods and was purified 32.72-fold with specific activity of 7988 U mg(-1). Fibrin zymography showed an active band, indicated acts as a plasmin-like protein with molecular weight of 72 kDa. Fibrinolytic enzyme have optimum pH of 6.0, stable in the range of 6.0 to 10.0 during 24 h and optimum temperature at 40 degrees C with a stability below 50 degrees C. Fibrinolytic enzyme is a serine metalloprotease by to be enhanced by Fe2+ and inhibited by PMSF. The enzyme has higher enzymatic activity than most other fibrinolytic enzymes and is stable at temperature and pH human physiological. Overall, the fibrinolytic enzyme from A. platensis has attractive biochemical properties to potential applications in the treatment of thrombosis. (C) 2020 Elsevier B.V. All rights reserved.
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