Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 89, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.intimp.2020.107045
Keywords
TREM-1; NLRP3 inflammasome; Acute lung injury; HMGB1; IL-18
Categories
Funding
- National Natural Science Foundation of China [81670014, 91949110]
- Hunan Provincial Natural Science Foundation of China [2019JJ50975]
- Research Foundation of Education Bureau of Hunan Province, China [18A491]
- Fundamental Research Funds for the Central Universities of Central South University [2020zzts219]
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NOD-, LRR- and pyrin domain-containing 3 (NLRP3) inflammasome and triggering receptor expressed on myeloid cells-1 (TREM-1) are considered critical orchestrators of the inflammatory response in acute lung injury (ALI). However, few assumptions are based on the relationship between them. Here, we investigated the effect of NLRP3 inflammasome activation on the TREM-1 expression in lipopolysaccharide (LPS)-induced ALI and macrophages. We found that inhibition of the NLRP3 inflammasome reduced the TREM-1 expression and pathological lung injury in mice with ALI. Then, primary murine macrophages were used to dissect the underlying mechanistic events of the activation NLRP3 inflammasome involved in the TREM-1 expression. Our results demonstrated that the conditioned medium (CM) from NLRP3 inflammasome-activated-macrophages up-regulated the TREM-1 expression in macrophages, while this effect was reversed by an NLRP3 inflammasome inhibitor MCC950. Furthermore, neutralizing antibodies anti-IL-18 and anti-HMGB1 reduced the TREM-1 expression induced by NLRP3 inflammasome activation. Mechanistically, we found that CM from NLRP3 inflammasome-activated-macrophages increased the level of inhibitor kappa B kinase protein phosphorylation (p-I kappa B alpha) and reactive oxygen species (ROS) content, and promoted I kappa B alpha protein degradation in macrophages. While the inhibition of nuclear factor kappa-B (NF-kappa B) and scavenging ROS eliminated the up-regulation of TREM-1 induced by the NLRP3 inflammasome activation in macrophages. In summary, our study confers NLRP3 inflammasome as a new trigger of TREM-1 signing, which allows additional insight into the pathological of the inflammatory response in ALI.
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