Journal
INSECT SCIENCE
Volume 28, Issue 6, Pages 1690-1707Publisher
WILEY
DOI: 10.1111/1744-7917.12880
Keywords
chitin‐ bindingDiaphorina citri; RNA interference; transcriptome sequencing; validamycin
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Funding
- Natural Science Foundation of Jiangxi Province [20202BAB215004]
- National Natural Science Foundation of China [31960116]
- Educational Commission of Jiangxi Province of China [GJJ180747]
- Key Research and Development Plan of Jiangxi Province [20192BBF60057, 2017ZDD02005]
- Ministry of Science and Technology of China [2018YFD0201504]
- earmarked fund for Jiangxi Agriculture Research System [JXARS-07]
- Gannan Normal University-level bidding project [20162B08]
- Graduate student innovation Fund Project [YC2019-S392]
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This study used comparative transcriptome analysis to reveal the effects of validamycin on D. citri, identifying two effective RNA interference targets.
Validamycin has been widely used as a specific competitive inhibitor of trehalase. In our previous research, validamycin significantly inhibited trehalase activity and chitin synthesis in Diaphorina citri, resulting in abnormal phenotypes. However, the mechanism of validamycin's action on D. citri remains unclear. Here, using a comparative transcriptome analysis, 464 differentially expressed genes (DEGs) in D. citri were identified after validamycin treatment. A Gene Ontology enrichment analysis revealed that these DEGs were mainly involved in small molecule process, structural molecule activity and transition metal ion binding. DEGs involved in chitin metabolism, cuticle synthesis and insecticide detoxification were validated by reverse transcription quantitative polymerase chain reaction. The RNA interference of D. citri chitinase-like protein ENO3 and D. citri cuticle protein 7 genes significantly affected D. citri molting. Moreover, the recombinant chitinase-like protein ENO3 exhibited a chitin-binding property, and an antimicrobial activity against Bacillus subtilis. This study provides a first insight into the molecular changes in D. citri after exposure to validamycin and identifies two effective RNA interference targets for D. citri control.
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