Spatial distribution and molecular epidemiology of hemotropic Mycoplasma spp. and Mycoplasma haemocanis infection in dogs from Rio de Janeiro, Brazil

This cross-sectional study aims to investigate the epidemiology and spatial distribution of hemotropic Mycoplasma spp. and Mycoplasma haemocanis in dogs from Rio de Janeiro, Brazil. Blood samples were collected at random from 437 household dogs. An epidemiological questionnaire was completed concerning the host characteristics as well as the environments in which they lived. A positivity frequency of 17.84% (78/437) was found for Mycoplasma spp. and 2% (9/437) for M. haemocanis in Rio de Janeiro, Brazil, through molecular detection based on the 16S rRNA sequence. According to the present study, dogs that live in households with the presence of rodents (odds ratio [OR] = 9.93; p-value = 0.02; confidence interval [CI]: 1.34-73.66) and wild animals (OR = 1.91; p-value = 0.03; CI: 1.06-3.42) are more likely to be infected with Mycoplasma spp.. Also, dogs with tick infestation (OR = 6.47; p-value = 0.007; CI: 1.63-25.60) have more chances to become infected with M. haemocanis. The spatial analysis disclosed a positive correlation between the Mycoplasma presence and tick infestation (global Moran index = 0.82; pseudo-p-value =0.001). The epidemiological findings support the hypothesis of Rhipicephalus sanguineus s.l. as the vector of M. haemocanis in the studied region and provide insightful information to prevent the Mycoplasma spp. infection in dogs from Rio de Janeiro

Automated summary

This study investigated the epidemiology and spatial distribution of hemotropic Mycoplasma spp. and Mycoplasma haemocanis in household dogs from Rio de Janeiro, Brazil, revealing infection rates and factors associated with the presence of these pathogens in dogs. The findings also suggest a potential vector for M. haemocanis and provide insights for prevention of Mycoplasma spp. infection in dogs in this region.