Journal
INDUSTRIAL CROPS AND PRODUCTS
Volume 158, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.indcrop.2020.113044
Keywords
Brewing industry waste; Microwave pretreatment; Xylooligosaccharides; ABE fermentation; Biorefinery
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Funding
- Regional Government of Castilla y Le PRIME
- EU-FEDER [VA010P17, CLU 2017-09, UIC 129]
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This study proposes an integrated biorefinery of brewer's spent grain (BSG) based on the application of a microwave pretreatment to obtain bioproducts, such as arabinoxylans (AX) and an advanced biofuel, such as biobutanol. A prehydrolysate with 17 g/L AX was obtained at 172 degrees C and 0.38 M NaOH, which were subsequently precipitated using an organosolvent process, recovering 133 kg AX/t BSG. The subsequent enzymatic hydrolysis with endo-xylanases significantly increased the concentration of oligomers with 2-6 units of xylose. The presence of oligosaccharides with a degree of polymerization (DP) from 3 to 27 was confirmed by MALDITOF MS. On the other hand, the pretreated solid residue obtained in the microwave assisted alkaline pretreatment was enzymatically hydrolyzed with cellulases and fermented by Clostridium beijerinckii, producing 9.9 g/L butanol (28 kg butanol/t BSG and 37 kg ABE/t BSG). Thus, the potential for the efficient use of BSG in an integrated biorefinery was demonstrated.
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