Regulation of circular RNAs act as ceRNA in a hypoxic pulmonary hypertension rat model

To explore potential critical genes and identify circular RNAs (circRNAs) that act as the competitive endogenous RNA (ceRNA) in a hypoxic pulmonary hypertension (HPH) rat model. Constructed rat model, and a bioinformatics method was used to analyse differentially expressed (DE) genes and construct a circRNA-miRNA-mRNA ceRNA regulatory network. Then, qRT-PCR was used to verify. The significant DEcircRNAs/DEmiRNAs/ DEmRNAs was showed, and a ceRNA network with 8 DEcircRNAs, 9 DEmiRNAs and 46 DEmRNAs were constructed. The functional enrichment suggested the inflammatory response, NF-kappa B signalling, MAPK cascade and Toll-like receptor were associated with HPH. Further assessment confirmed that circ_002723, circ_008021, circ_016925 and circ_020581 could have a potential ceRNA mechanism by sponging miR-23a or miR-21 to control downstream target gene and be involved in the pathophysiology of HPH. The qRT-PCR validation results were consistent with the RNA-Seq results. This study revealed potentially important genes, pathways and ceRNA regulatory networks in HPH.

Automated summary

This study identified potential critical genes and circRNAs acting as ceRNAs in a HPH rat model. A ceRNA network was constructed, showing association with inflammatory response, NF-kappa B signaling, MAPK cascade, and Toll-like receptor. Certain circRNAs could potentially control downstream target genes by sponging miR-23a or miR-21 in the pathophysiology of HPH, as confirmed by qRT-PCR validation.