Journal
GENOMICS
Volume 113, Issue 1, Pages 151-158Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2020.12.001
Keywords
Freeze-thaw resistant; Freeze-resistant; UV-C resistant; Molecular cold-adaptation; Cold-adaptation score; Aromaticity index
Funding
- DST, Govt. of India [DST/INSPIRE/04/2014/001280]
- Science and Engineering Research Board [SRG/2019/001071]
- NMHS project of MoEFCC [GBPNI/NMHS-2018-19/SG/178]
- DST-TDT [DST/TDT/WM/2019/43]
- Council of Scientific & Industrial Research (CSIR), Government of India [MLP-0143, MLP-0137, MLP-0201]
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The study selected two strains of Arthrobacter with efficient survivability under high altitude stress conditions for comparative genomic studies. Physiological analysis and genome insights supported the survival of these strains under multiple high-altitude stress conditions. The molecular cold adaptation analysis showed the incidence of more cold-adapted proteins and favored amino acids for broad temperature survivability.
Arthrobacter is a dominant aerobic bacterium under the class Actinobacteria, known for its nutritionally versatile nature and wide prevalence in stressful environments. In the current study representative two strains of Arthrobacter, ERGS1:01 and ERGS4:06, with efficient survivability under high altitude stress conditions were selected for comparative genomic studies with their mesophilic counterparts. Physiological analysis and genome insights supported the survival of these strains under multiple high-altitude stress conditions. Molecular cold adaptation and substitution analysis of the studied strains supported the incidence of more cold-adapted proteins for functionality at low temperatures. Studied strains preferred amino acids like serine, asparagine, lysine, tryptophan for favoring increased flexibility supporting their broad temperature survivability. To the best of our knowledge, this is the first molecular cold adaptation analysis performed for the genus Arthrobacter and has revealed that 'aromaticity', one of the cold-adaptor indicators, should be carefully considered while evaluating cold adaptation strategies in psychrotrophic/psychrophilic bacteria.
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