Journal
FREE RADICAL RESEARCH
Volume 55, Issue 1, Pages 11-25Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10715762.2020.1854455
Keywords
Aβ (1-42)-induced memory impairment; GPx-1 knockout mice; GPx-1 gene-encoded adenovirus vector; M1 mAChR; CREB; BDNF; Hippocampus
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Funding
- Kuhnil Pharmaceutical Co., Ltd., Republic of Korea
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT, Republic of Korea [NRF-2019R1I1A3A01063609, NRF-2019R1A2C4070161]
- BK21 PLUS program through the National Research Foundation of Korea (NRF) - Ministry of Education of Korea
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The study demonstrated that the GPx-1 gene protects against A beta (1-42)-induced memory impairments by activating the M1 mAChR-dependent CREB/BDNF signaling pathway.
In the present study, we examined whether glutathione peroxidase-1 (GPx-1), a major H2O2 scavenger in the brain, affects memory deficits induced by A beta (1-42) in mice. Treatment with 400 pmol/5 mu l A beta (1-42) (i.c.v.) resulted in a reduction of GPx-1 expression in wild-type (WT) mice. An A beta (1-42)-induced reduction in acetylcholine (ACh) level was observed in the hippocampus. Treatment with A beta (1-42) consistently resulted in reduced expression and activity of choline acetyltransferase (ChAT) and in an increase in expression and activity of acetylcholinesterase (AChE). Upon examining each of the muscarinic acetylcholine receptors (mAChRs) and nicotinic AChRs, we noted that A beta (1-42) treatment selectively reduced the levels of M1 mAChR. In addition, A beta (1-42) induced a significant reduction in phospho-cAMP response element-binding protein (p-CREB) and brain-derived neurotrophic factor (BDNF) expression. The cholinergic impairments induced by A beta (1-42) were more pronounced in GPx-1 knockout mice than in WT mice. Importantly, an adenoviral vector encoded with the GPx-1 gene (Ad-GPx-1) significantly rescued A beta (1-42)-induced cholinergic impairments in GPx-1 knockout mice. In addition, M1 mAChR antagonist dicyclomine significantly counteracted Ad-GPx-1-mediated increases in p-CREB and BDNF expression, as well as memory-enhancing effects in GPx-1 knockout mice, thus indicating that M1 mAChR might be a critical mediator for the rescue effects of Ad-GPx-1. Combined, our results suggest that GPx-1 gene protected against A beta (1-42)-induced memory impairments via activation of M1 mAChR-dependent CREB/BDNF signalling.
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