Novel monoclonal antibody-sandwich immunochromatographic assay based on Fe3O4/Au nanoparticles for rapid detection of fish allergen parvalbumin

In this study, a rapid sandwich immunochromatographic assay (ICA) was developed to detect parvalbumin (PV). Firstly, two optimum primary monoclonal antibody (mAb) against PV had been screened out: mAb1 was used as the capture antibody, and mAb2 conjugated to Fe3O4/Au nanoparticles (Fe3O4/AuNPs) that served as a detection reagent. Using this pair of mAbs, a sandwich ICA strip based on Fe3O4/AuNPs was developed. The results showed that the color intensity of test line positively correlated with the PV concentration in the standard or spiked sample. The limit of detection for qualitative (LOD) and quantitative detection (LOQ) were 2 ng/mL and 0.691 ng/mL, respectively. Besides, the detection time of this ICA strip was within 15 min. The recovery rates ranged from 104.0% to 117.4%, within an acceptable level (80?120%). Moreover, the developed assay also showed high cross reaction in different fish species. These results demonstrated that the established test strip has the potential to be used as a rapid screening tool for large scale determination of PV in foodstuffs.

Automated summary

A rapid sandwich immunochromatographic assay was developed to detect parvalbumin (PV) using two optimal PV antibodies, resulting in a strip with Fe3O4/AuNPs. The method showed low detection limits, short detection time, and high cross-reactivity in different fish species, indicating its potential as a rapid screening tool for large-scale determination of PV in foodstuffs.