4.5 Article

The reproductive regulation of LPXRFa and its receptor in the hypothalamo-pituitary-gonadal axis of the spotted scat (Scatophagus argus)

Journal

FISH PHYSIOLOGY AND BIOCHEMISTRY
Volume 47, Issue 1, Pages 93-108

Publisher

SPRINGER
DOI: 10.1007/s10695-020-00898-2

Keywords

Hypothalamo-pituitary-gonadal (HPG) axis; LPXRFa; Neuropeptide; Receptor; Scatophagus argus

Funding

  1. National Natural Science Foundation of China [31972775]
  2. Open project fund of the Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education)
  3. Key Laboratory of Aquatic Science of Chongqing, College Students' innovation and Entrepreneurship Project [CXXL2018132]
  4. Natur a l Science Foundation of Guangdong Province [2017A030313101]
  5. Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams [2019KJ149]
  6. Department of Education of Guangdong Province [2018KTSCX090, 2018KQNCX106]

Ask authors/readers for more resources

The study found that Lpxrfa in spotted scat may play a role in regulating reproduction and have significant impact on the hypothalamo-pituitary-gonadal axis.
Gonadotropin-inhibitory hormone (GnIH) plays a critical role in regulating gonadotropin-releasing hormone (GnRH), gonadotropin hormone (GtH), and steroidogenesis. The Lpxrfa (the piscine ortholog of GnIH) system has been found to regulate fish reproduction. To gain insight into the role of Lpxrfa in the regulation of spotted scat (Scatophagus argus) reproduction, spotted scat Lpxrfa (ssLpxrfa), and its receptor (ssLpxrfa-r) were cloned and analyzed. Tissue distribution and expression patterns at the hypothalamo-pituitary-gonadal axis (HPG axis) of sslpxrfa and sslpxrfa-r mRNA were also investigated during gonadal development of spotted scat. The open reading frame (ORF) of the sslpxrfa was 606 bp encoding 201 amino acids and includes a putative signal peptide and two mature ssLpxrfa peptides with LPXRFamide motif at their C-terminus. The sslpxrfa-r ORF was 1449 bp encoding 482 amino acids and contracted a seven-hydrophobic transmembrane (TM) domain structure. The tissue distribution showe d that the sslpxrfa was highly expressed in hypothalami, gill, and the gonads. In addition, sslpxrfa-r was highly expressed in hypothalami, pituitaries, and the gonads. Quantitative real-time polymerase chain reaction (qPCR) revealed that sslpxrfa had the highest expression in the hypothalami and pituitaries, and the lowest expression in the gonads in stage V. During gonadal development, the expression of sslpxrfa-r was gradually increased in the hypothalami but reduced in the gonads. However, no obvious trend was observed in the pituitaries. The expression of sslpxrfa and sslpxrfa-r decreased significantly after injection with 17 beta-estradiol (E-2). However, the expression of both sslpxrfa and sslpxrfa-r was not changed after injection with 17 alpha-methyltestosterone(17 alpha-MT) in the hypothalami. In addition, no changes were observed in the expression of fsh beta and lh beta in the pituitaries after injecting ssLpxrfa-1. However, ssLpxrfa-2 could downregulate the expression of sbgnrh and fsh beta in the hypothalami and pituitaries, respectively. Taken together, these findings suggested that ssLpxrfa may participate in E-2 feedback in reproduction and regulate the reproductive axis of spotted scat.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available