Journal
FEBS LETTERS
Volume 594, Issue 23, Pages 3815-3838Publisher
WILEY
DOI: 10.1002/1873-3468.13997
Keywords
ABC transporters; degenerate nucleotide binding site; transport cycle model; type I exporter
Funding
- Austrian Science Fund [FWF-SFB035-24]
- Austrian Science Fund (Stand-Alone Project) [32017]
- DFG [CRC 1208, Schm1279/13-1 CRC 1208, Schm1279/13-1]
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ATP-binding cassette (ABC) transporters are fascinating molecular machines that are capable of transporting a large variety of chemically diverse compounds. The energy required for translocation is derived from binding and hydrolysis of ATP. All ABC transporters share a basic architecture and are composed of two transmembrane domains and two nucleotide binding domains (NBDs). The latter harbor all conserved sequence motifs that hallmark the ABC transporter superfamily. The NBDs form the nucleotide binding sites (NBSs) in their interface. Transporters with two active NBSs are called canonical transporters, while ABC exporters from eukaryotic organisms, including humans, frequently have a degenerate NBS1 containing noncanonical residues that strongly impair ATP hydrolysis. Here, we summarize current knowledge on degenerate ABC transporters. By integrating structural information with biophysical and biochemical evidence of asymmetric function, we develop a model for the transport cycle of degenerate ABC transporters. We will elaborate on the unclear functional advantages of a degenerate NBS.
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