Journal
EXPERIMENTAL BIOLOGY AND MEDICINE
Volume 246, Issue 8, Pages 897-905Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/1535370220981859
Keywords
Infantile hemangioma; miR-200c-3p; notch signaling pathway; cell proliferation
Categories
Funding
- National Natural Science Foundation of China [8197102295, U1732157]
- Key Research and Development Project of Anhui Province [202004j07020034]
- National College Student Innovation Training Project of Anhui Medical University [201910366002]
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MiR-200c-3p is downregulated in hemangioma tissue and promotes vascular endothelial cell proliferation by targeting the Notch signaling pathway, suggesting it plays a key role in hemangioma pathogenesis.
Excessive proliferation of vascular endothelial cells can cause hemangioma. Although typically benign, hemangiomas can become life-threatening. The microRNA miR-200c-3p is abnormally expressed in some types of tumors, but its expression, biological role, and mechanism of action in infantile hemangioma remain to be fully elucidated. The expression levels of miR-200c-3p in hemangioma tissue were compared with those in adjacent healthy tissue by using bioinformatics analyses and TargetScan. Western blot, enzyme-linked immunosorbent assay, and Cell Counting Kit 8 analyses were used to determine the biological function and site of action of miR-200c-3p in human dermal microvascular endothelial cells (HDMECs). MiR-200c-3p was one of the top 10 differentially expressed genes between healthy tissue, and hemangiomas tissues, having markedly decreased expression in hemangioma tissue. Reduction of miR-200c-3p expression in HDMECs through the transfection of a miR-200c-3p inhibitor significantly increased HDMEC proliferation. The addition of the Notch signaling pathway inhibitor DAPT to HDMECs transfected with the miR-200c-3p inhibitor eliminated the inhibitor-induced enhancement of proliferation in HDMECs. These findings indicate that miR-200c-3p targets the Notch signaling pathway to promote the proliferation of vascular endothelial cells, suggesting that miR-200c-3p plays an important role in the pathogenesis of hemangioma.
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