DNA hybridisation kinetics using single-molecule fluorescence imaging

Deoxyribonucleic acid (DNA) hybridisation plays a key role in many biological processes and nucleic acid biotechnologies, yet surprisingly there are many aspects about the process which are still unknown. Prior to the invention of single-molecule microscopy, DNA hybridisation experiments were conducted at the ensemble level, and thus it was impossible to directly observe individual hybridisation events and understand fully the kinetics of DNA hybridisation. In this mini-review, recent single-molecule fluorescence-based studies of DNA hybridisation are discussed, particularly for short nucleic acids, to gain more insight into the kinetics of DNA hybridisation. As well as looking at single-molecule studies of intrinsic and extrinsic factors affecting DNA hybridisation kinetics, the influence of the methods used to detect hybridisation of single DNAs is considered. Understanding the kinetics of DNA hybridisation not only gives insight into an important biological process but also allows for further advancements in the growing field of nucleic acid biotechnology.

Automated summary

The study of DNA hybridization kinetics is crucial in understanding biological processes and advancing nucleic acid biotechnology. Single-molecule fluorescence techniques have provided new insights into the kinetics of DNA hybridization, shedding light on both intrinsic and extrinsic factors influencing the process. Additionally, the methods used to detect single DNA hybridization events play a significant role in the outcome of the research.