Reproductive toxicity of Roundup WG® herbicide: impairments in ovarian follicles of model organism Danio rerio

Glyphosate-based herbicides are widely used in global agriculture, and their effects on different non-target animal organisms have been the focus of many toxicological studies. Regarding the potential role of glyphosate-based herbicides as an endocrine disruptor, the present study aims to investigate the effects of the herbicide Roundup WG (R) (RWG) on female reproduction, specifically on the ovarian maturation of Danio rerio. Adult females were exposed to low concentrations of RWG (0.065, 0.65, and 6.5 mg L-1) for 15 days, and then the ovaries were submitted to structural and morphometric procedures, accompanied by analysis of the vitellin protein content. Our results showed an increase of initial ovarian follicle numbers, decrease of late ovarian follicles, and smaller diameter of ovarian follicles in fish exposed to 0.065 and 6.5 mg L-1. The thickness of vitelline envelope was reduced, and the vitellin protein content was increased in the ovarian follicle in the two highest concentrations. Ultrastructural changes in the ovarian follicular component were evident and expressed by the cell index; vacuolization in follicular cells, increase of perivitelline space, and impaired mitochondria in oocytes were observed. Therefore, RWG adversely affects the ovarian maturation in D. rerio, and these changes can lead to reproductive toxicity, compromising population dynamics.

Automated summary

Glyphosate-based herbicides have been widely used in global agriculture, and their potential impact as an endocrine disruptor on non-target animal organisms has been studied. This research specifically investigated the effects of the herbicide Roundup WG (R) on the ovarian maturation of Danio rerio, finding adverse effects on ovarian follicle numbers, thickness of vitelline envelope, and ultrastructural changes in follicular components, which could lead to reproductive toxicity in fish populations.