Journal
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 28, Issue 14, Pages 17132-17145Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-020-12152-6
Keywords
Metatranscriptomics; Functional metagenomics; Metal tolerance; Type I Hsp40; Metal uptake; Cadmium; Yeast complementation
Categories
Funding
- IFCPAR [4709-1]
Ask authors/readers for more resources
Using a functional metagenomics approach, a potentially toxic metal tolerance gene was identified from contaminated soil community, with the specific gene PLCc43 showing tolerance to various metals and oxidative stress. This gene may serve as a biomarker for detoxification of toxic metals in contaminated sites.
Pollution in the environment due to accumulation of potentially toxic metals results in deterioration of soil and water quality, thus impacting health of all living organisms including microbes. In the present investigation, a functional metagenomics approach was adopted to mine functional genes involved in metal tolerance from potentially toxic metal contaminated site. Eukaryotic cDNA library (1.0-4.0 kb) was screened for the genes providing tolerance to cadmium (Cd) toxicity through a functional complementation assay using Cd-sensitive Saccharomyces cerevisiae mutant ycf1(Delta). Out of the 98 clones able to recover growth on Cd-supplemented selective medium, one clone designated as PLCc43 showed more tolerance to Cd along with some other clones. Sequence analysis revealed that cDNA PLCc43 encodes a 284 amino acid protein harbouring four characteristic zinc finger motif repeats (CXXCXGXG) and showing partial homology with heat shock protein (Hsp40) of Acanthamoeba castellanii. qPCR analysis revealed the induction of PLCc43 in the presence of Cd, which was further supported by accumulation of Cd in ycf1(Delta)/PLCc43 mutant. Cu-sensitive (cup1(Delta)), Zn-sensitive (zrc1(Delta)) and Co-sensitive (cot1(Delta)) yeast mutant strains were rescued from sensitivity when transformed with cDNA PLCc43 indicating its ability to confer tolerance to various potentially toxic metals. Oxidative stress tolerance potential of PLCc43 was also confirmed in the presence of H2O2. Present study results suggest that PLCc43 originating from a functional eukaryotic gene of soil community play an important role in detoxification of potentially toxic metals and may be used as biomarker in various contaminated sites.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available