Nanocolloids, but Not Humic Acids, Augment the Phytotoxicity of Single-Layer Molybdenum Disulfide Nanosheets

Engineered nanomaterials (ENMs), especially transition metal dichalcogenide (TMDC), have received great attention in recent years due to their advantageous properties and applications in various fields and are inevitably released into the environment during their life cycle. However, the effect of natural nanocolloids, widely distributed in the aquatic environment, on the environmental transformation and ecotoxicity of ENMs remains largely unknown. In this study, the effects of natural nanocolloids were compared to humic acid on the environmental transformation and ecotoxicity of single-layer molybdenum disulfide (SLMoS2), a representative TMDC. SLMoS2 with nanocolloids or humic acid (HA) enhanced their dispersion and Mo ion release in deionized water. Nanocolloids induced growth inhibition, reactive oxygen species (ROS) elevation, and cell permeability. Low-toxicity SLMoS2 combined with nanocolloids will enhance the above adverse effects. SLMoS2-nanocolloids induced serious damage (cell distortion and deformation), SLMoS2 internalization, and metabolic perturbation on Chlorella vulgaris (C. vulgaris). In contrast, the addition of HA induced the growth promotion and lower ROS level, inhibited the internalization of SLMoS2, and mitigated metabolic perturbation on C. vulgaris. This work provides insights into the effect of natural nanocolloids on the behaviors and biological risks of ENMs in aquatic environments, deserving substantial future attention.

Automated summary

Engineered nanomaterials (ENMs), especially transition metal dichalcogenide (TMDC), have advantageous properties and applications in various fields. This study compared the effects of natural nanocolloids and humic acid on the environmental transformation and ecotoxicity of single-layer molybdenum disulfide (SLMoS2). The presence of natural nanocolloids significantly impacted the dispersion and biological risks of TMDC in aquatic environments, highlighting the need for further research in this area.