Journal
DEVELOPMENTAL BIOLOGY
Volume 419, Issue 1, Pages 114-120Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2016.03.018
Keywords
Flower; Flower development; Flower meristem; Plant development; Confocal microscopy; Live confocal imaging; Sepals; Floral organs
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Funding
- Howard Hughes Medical Institute
- US National Institutes of Health [R01 GM104244]
- Gordon and Betty Moore Foundation [GBMF3406]
- US National Science Foundation [IOS-0926347]
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Recent advances in confocal microscopy, coupled with the development of numerous fluorescent reporters, provide us with a powerful tool to study the development of plants. Live confocal imaging has been used extensively to further our understanding of the mechanisms underlying the formation of roots, shoots and leaves. However, it has not been widely applied to flowers, partly because of specific challenges associated with the imaging of flower buds. Here, we describe how to prepare and grow shoot apices of Arabidopsis in vitro, to perform both single-point and time-lapse imaging of live, developing flower buds with either an upright or an inverted confocal microscope. (C) 2016 Elsevier Inc. All rights reserved.
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