Microbial community shift via black carbon: Insight into biological nitrogen removal from microbial assemblage and functional patterns

Understanding the ecological relationship of microbial community under external stimulation is crucial for environmental restoration. Black carbon (e.g., biochar) have been widely deemed as a strategy to enhance pollutants removal because of its structure and redox-active property. However, the underlying ecological mechanism of microbial community under black carbon addition is poorly understood. The major purposes of this study were to determine the microbial assemblage and functional patterns via null model test, network analysis, and function prediction. The results showed that the nitrate removal efficiency of modified black carbon system achieved 46.44%. Both deterministic and stochastic processes were significant for mediating the microbial assemblage and the deterministic process dominated (>95%) the assemblage of community. Keystone taxa in the black carbon systems, involving Sulfuricella, Allorhizobium, and Nitrospira, stimulated the shift of community composition regarding the nitrogen removal. The existence of black carbon and the biotic interactions increased biological nitrate utilization and promoted nitrogen removal. Overall, this study presents the mechanism of bacterial community assembly and provides insight into biological nitrogen removal from microbial ecological perspective.

Automated summary

This study aims to determine the microbial assemblage and functional patterns under black carbon addition, and found that the nitrate removal efficiency of modified black carbon system achieved 46.44%. The deterministic process dominated the assemblage of community, with key taxa like Sulfuricella, Allorhizobium, and Nitrospira stimulating the shift of community composition regarding nitrogen removal.