Journal
ENVIRONMENTAL POLLUTION
Volume 269, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2020.116112
Keywords
Marsupenaeus japonicas; Nrf2-Keap1 pathway; Oxidative stress; Detoxification; Apoptosis
Categories
Funding
- project of agricultural improved variety project in Shandong Province-Breeding of breakthrough new prawn varieties with high quality and resistance [2019LZGC014]
- Projects of International Exchange and Cooperation in Agriculture, Ministry of Agriculture and Rural Affairs of China-Science
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The study revealed the role of Nrf2 in Marsupenaeus japonicas in response to cadmium exposure, with Nrf2 silence leading to increased oxidative stress and apoptosis, while SFN treatment reduced oxidative stress and apoptosis. These findings indicate the significant involvement of the Nrf2 pathway in Cd-induced cellular oxidative damage in M. japonicus.
Nuclear factor-erythroid 2-related factor-2 (Nrf2) is an important modulator of cellular responses against Cd in mammalian cells. However, whether such modulation is conserved in Marsupenaeus japonicas remains unknown. In our study, the shrimps were injected with dsRNA targeting Nrf2 at 4 mu g g(-1) body weight (b.w.) or sulforaphane (SFN) at 5 mu g g(-1) b.w., and then were exposed to 40 mg L-1 CdCl2 for 48 h. After Nrf2 knockdown, the Cd content increased, but decreased in the SFN group. This suggested that Nrf2 could promote Cd excretion. A terminal deoxynulceotidyl transferase nick-end-labeling (TUNEL) assay revealed that the Nrf2 knockdown increased the number of apoptotic cells in M. japonicas, while SFN decreased the number of apoptotic cells. After Nrf2 knockdown, the total antioxidant capacity (T-AOC), superoxide dismutase (Sod) activity, and related gene expression decreased significantly, while the malondialdehyde (MDA) content increased remarkably. By contrast, SFN injection alleviated the oxidative stress, as evidenced by increased T-AOC, Sod activity, sod mRNA expression and a reduced MDA content. Similarly, detoxification related enzyme activities (ethoxyresorufin O-deethylase and glutathione-S-transferase (GST)) and their corresponding gene expressions (cyp3a (cytochrome P450 family 3 subfamily A) and gst) were suppressed in the ds-Nrf2 injection group, while they were elevated in the SFN group. In addition, ds-Nrf2 activated mitochondrial apoptotic pathway, as evidenced the mRNA and protein levels of caspase-3, Bcl2 associated X protein (Bax), and p53, while SFN treatment suppressed them. These results displayed that in M. japonicus Cd-induced cellular oxidative damage probably acts via the Nrf2 pathway. (C) 2020 Elsevier Ltd. All rights reserved.
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