Wastewater remediation of heavy metals and pesticides using rice straw and/or zeolite as bioadsorbents and assessment of treated wastewater reuse in the culture of Nile tilapia (Oreochromis niloticus)

The remediation of wastewater (WW) is a promising solution for limited water sources. This study aimed to evaluate rice straw (RS) and zeolite (Z) as bioadsorbents for the removal of pollutants, including heavy metals (HMs) (cadmium [Cd], nickel [Ni], and lead [Pb]) and malathion (PC), from WW and to assess the suitability of reusing remediated WW in fish rearing units. A total of 11 treatment groups with 3 replicates each were designed with different combinations of RS and/or Z for the treatment of real WW contaminated with HMs and malathion, where the WW remained in contact with the adsorbents for 24 h. Different remediated WWs were used for rearing Nile tilapia (Oreochromis niloticus), which were randomly allocated into 33 glass aquaria representing 11 treatments with 3 replicates each for 30 days. The best remediation efficiency was achieved using a mixture of whole RS (WRS), chopped RS (CRS), and Z (HM-PC-WRS-CRS-Z group), with removal percentages of 92%, 95%, 96%, and 99% for Cd, Ni, Pb, and malathion, respectively. The health status of the aquatic ecosystems was assessed through blood tests to characterize biochemical parameters and through pathological changes of cultured O. niloticus reared in treated WW. A significant (P < 0.05) effect on the blood biochemistry of fish reared in treated WW was found and better biochemical and histologic architecture was observed than that of fish reared in untreated WW. A novel mixture of WRS, CRS, and Z could possibly be a promising low-cost adsorbent for wastewater treatment.