Isolation and genomic characterization of the ibuprofen-degrading bacterium Sphingomonas strain MPO218

The presence of pharmaceutical compounds in waters and soils is of particular concern because these compounds can be biologically active, even at environmental concentrations. Most pharmaceutical contaminants result from inefficient removal of these compounds during wastewater treatment. Although microorganisms able to biodegrade pharmaceuticals compounds have been described, the isolation and characterization of new bacterial strains capable of degrading drugs remain important to improve the removal of this pollutant. In this work, we describe the Sphingomonas wittichii strain MPO218 as able to use ibuprofen as the sole carbon and energy source. The genome of MPO218 consists of a circular chromosome and two circular plasmids. Our analysis shows that the largest plasmid, named pIBU218, is conjugative and can horizontally transfer the capability of growing on ibuprofen after conjugation with another related bacterium, Sphingopyxis granuli TFA. This plasmid appears to be unstable since it undergoes different deletions in absence of selection when growth on ibuprofen is not selected. This is the first described example of a natural and conjugative plasmid that enables growth on ibuprofen and is another example of how horizontal gene transfer plays a crucial role in the evolution of bacteria.

Automated summary

The presence of pharmaceutical compounds in waters and soils is a concern due to their potential biological activity, even at low environmental concentrations. The study identified Sphingomonas wittichii strain MPO218 as capable of utilizing ibuprofen as the sole carbon and energy source, and its plasmid pIBU218 as conjugative and able to transfer the ability to grow on ibuprofen to other bacteria. This research highlights the importance of horizontal gene transfer in bacterial evolution and the potential for improving pharmaceutical compound removal.