Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 65, Issue -, Pages 124-131Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2016.07.001
Keywords
Insect; Silkworm; Innate immunity; Apolipophorin; Prophenoloxidase; Antimicrobial peptides
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Funding
- National Natural Science Foundation of China [31501903, 30972770, 31100647]
- Liaoning Key Laboratory of Pattern Recognition
- Shenyang Key Laboratory of Medical Devices [F13-292-1-00]
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Apolipophorin-III (apoLp-III) is an exchangeable apolipoprotein found in many insect species and functions as a lipid transport vehicle. Recent studies have shown that apoLp-III is a multifunctional molecule involved in not only lipid transportation but also innate immune responses. In the present study, the pattern recognition properties of Antheraea pernyi apoLp-III were investigated. Recombinant Ap-apoLp-III was bound to different species of microbes and further study showed the rAp-apoLp-III is capable of interacting with pathogen associated molecular patterns (PAMPs) on the microbial cell surface. In addition, an Ap-apoLp-III/PAMP mixture stimulated the prophenoloxidase (PPO) activation of A. pernyi hemolymph in vitro, to a greater extent than PAMP alone while Ap-apoLp-III itself failed to activate the PPO system, indicating that Ap-apoLp-III up-regulates PPO activation by combining with PAMP. After pathogen invasion following an injection of Staphylococcus aureus, RNAi-mediated silencing of apoLp-III decreased the transcriptional abundance of three antimicrobial peptide genes. These data suggest that apoLp-III is a versatile pattern recognition receptor and may play important roles in the innate immune responses of Antheraea pernyi. (C) 2016 Published by Elsevier Ltd.
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