Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 55, Issue -, Pages 1-11Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2015.10.004
Keywords
TNF ligands; B cell activating factor (BAFF); Immune responses; Recombinant protein; Lymphocyte viability
Categories
Funding
- Ministry of Oceans and Fisheries, Korea
- Basic Science Research Program through the National Research Foundation of Korea (NRF) by the Ministry of Education [2013R1A1A2064735]
- National Research Foundation of Korea [2013R1A1A2064735] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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B cell activating factor (BAFF) is a member of the tumor necrosis factor (TNF) ligand family. BAFF has been shown to induce survival and proliferation of lymphocytes. We characterized the gene encoding BAFF (RbBAFF) in rock bream (Oplegnathus fasciatus), and attempted to determine its biological functions upon immune responses. In silk analysis of RbBAFF demonstrated the presence of common TNF ligand family features, including a TNF domain, a D-E loop, and three cysteine residues that are crucial for trimer formation. Amino acid sequence alignment confirmed that RbBAFF and its homologs were conserved at secondary and tertiary levels. Transcriptional analysis indicated that RbBAFF mRNAs were ubiquitously expressed in wide array of tissues. The higher levels of constitutive expression were observed in the kidney, head kidney and spleen, suggesting an important physiological relationship with lymphocytes. Under pathological conditions, RbBAFF mRNA levels were significantly elevated. The role of RbBAFF in lymphocyte survival and proliferation was confirmed by MIT assays and flow cytometry. Recombinant RbBAFF protein (10 mu g/mL) was able to prolong the survival and/or enhance the proliferation of rock bream lymphocytes by approximately 30%. Transcription of IL-10 and NF kappa B-1 was significantly stimulated by RbBAFF. Our findings provide further information regarding fish BAFF gene and its role in adaptive immunity. (C) 2015 Elsevier Ltd. All rights reserved.
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