Journal
DEVELOPMENT GROWTH & DIFFERENTIATION
Volume 58, Issue 7, Pages 600-608Publisher
WILEY
DOI: 10.1111/dgd.12301
Keywords
male infertility; sperm count; spermatogenesis; testis; zinc finger
Categories
Funding
- Ministry of Education, Science, Sports, and Culture of Japan
- Promotion and Mutual Aid Corporation for Private Schools of Japan
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Zfp318, a mouse gene with a Cys2/His2 zinc finger motif, is mainly expressed in germ cells in the testis. It encodes two alternative transcripts, which regulate androgen receptor-mediated transcriptional activation or repression by overexpression of them. However, the role of Zfp318 is still obscure invivo, especially in spermatogenesis. To elucidate the role of Zfp318 during gamete production, we established a knockout mouse line. Zfp318-null male mice exhibited infertility, whereas Zfp318-null female mice displayed normal fertility. ZFP318 was expressed during multiple stages of spermatogenesis, from spermatocytes to round spermatids. The nuclei of secondary spermatocytes showed high levels of expression. Histological analysis and quantitative analysis of DNA content showed decreased numbers of both spermatids in the seminiferous tubules andmature spermatozoa in the epididymides of Zfp318-null mice. These results suggest that Zfp318 isexpressed as a functional protein in testicular germ cells and plays an important role in meiosis during spermatogenesis.
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