Journal
DEVELOPMENT
Volume 143, Issue 12, Pages 2194-2205Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.134981
Keywords
Dishevelled antagonist of beta-catenin; Dapper; Frodo; beta-catenin; Nuclear bodies; Xenopus embryo; Chick embryo
Categories
Funding
- Medical Research Council [MR/J000655/1, MR/M010465]
- Biotechnology and Biological Sciences Research Council (BBSRC) [M008517]
- Ministerio de Economia y Competitividad [BFU2011-24099, BFU2013-46477-P, BFU2014-55738-REDT]
- MRC [MR/J000655/1] Funding Source: UKRI
- Medical Research Council [MR/J000655/1] Funding Source: researchfish
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Delamination of neural crest (NC) cells is a bona fide physiological model of epithelial-to-mesenchymal transition (EMT), a process that is influenced by Wnt/beta-catenin signalling. Using two in vivo models, we show that Wnt/beta-catenin signalling is transiently inhibited at the time of NC delamination. In attempting to define the mechanism underlying this inhibition, we found that the scaffold proteins Dact1 and Dact2, which are expressed in pre-migratory NC cells, are required for NC delamination in Xenopus and chick embryos, whereas they do not affect the motile properties of migratory NC cells. Dact1/2 inhibit Wnt/beta-catenin signalling upstream of the transcriptional activity of T cell factor (TCF), which is required for EMT to proceed. Dact1/2 regulate the subcellular distribution of beta-catenin, preventing beta-catenin from acting as a transcriptional coactivator to TCF, yet without affecting its stability. Together, these data identify a novel yet important regulatory element that inhibits beta-catenin signalling, which then affects NC delamination.
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