Journal
ELECTROCHIMICA ACTA
Volume 362, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2020.137168
Keywords
Electrochemical; Intercalation; MicroRNA; Signal amplification
Categories
Funding
- National Natural Science Foundations of China [81571812]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [1107047002]
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MicroRNAs (miRNAs) are becoming biomarkers for the clinical diagnosis of many diseases. However, their intrinsic properties such as low enrichment and short sequence restrict their applications. Here, we designed an ultrasensitive dual signal amplification electrochemical biosensor based on sodium alginate-polypyrrole/Au nanoparticles (SA-PPy/AuNPs) nanocomposite for the detection of miRNAs. Firstly, a planar intercalated copper(II) complex was synthesized to be the signal molecule. Then, catalytic hairpin assembly (CHA) reaction and catalytic reaction between ferric ion and copper ion were combined as a dual signal amplification to improve the sensitivity of the biosensor. In the presence of target miRNAs, multiple double helix structure formed for the CHA reaction was triggered, which immobilized more copper(II) complex on the surface of the working electrode and a strong signal was obtained in the square wave voltammetry (SWV) test. Also, the signal can be re-amplified again in the presence of ferric ion in the electrolyte. Under the optimal conditions, based on these strategies, a sensitive electrochemical biosensor was constructed for the detection of miRNAs in the range of 1 fM to 1 nM and the limit of detection was as low as 0.34 fM. Furthermore, the developed biosensor with excellent selectivity and reproducibility tested on human serum samples shows broad application prospects in the early stage of tumor diagnosis. (C) 2020 Elsevier Ltd. All rights reserved.
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