Hemin-phytic Acid Functionalized Porous Conducting Polymer Hydrogel With Good Biocompatibility for Electrochemical Detection of H2O2 Released From Living Cells

A novel hemin/phytic acid doped polyaniline (PA-PANI) hydrogel composite was prepared through a simple chemical and self-assembly method, which was modified onto electrode for electrochemical detection of H2O2 released from living cells. It showed good analytical performance with high sensitivity, selectivity and a rapid response for the analysis of H2O2 in the range of 2 to 102 mu M, with the detection limit of 1.2 mu M. The favorable results mainly originated from both the high conductivity of PA-PANI hydrogel and its network structure preventing hemin from self-dimerization to provide active catalytic species. Furthermore, PA-PANI with good biocompatibility allowed living cells to adhere and resulted in a short diffusion distance between H2O2 released from cells and electrode.

Automated summary

The novel hemin/phytic acid doped polyaniline (PA-PANI) hydrogel composite prepared through a simple chemical and self-assembly method showed good analytical performance for electrochemical detection of H2O2 released from living cells. The high sensitivity, selectivity and rapid response were attributed to the high conductivity and network structure of PA-PANI hydrogel, as well as its prevention of hemin self-dimerization. The biocompatibility of PA-PANI allowed living cells to adhere and facilitated a short diffusion distance between H2O2 released from cells and electrode.